Organoids

• Our company utilizes a proprietary iPSC technology platform to successfully achieve stable production of 3D cardiac organoids, which can autonomously contract within six days.
• The production of our cardiac organoids achieves 100% differentiation efficiency, with all differentiation reagents and drugs being self-developed and self-produced.
• The cost is only one-quarter of the corresponding Thermo-Fisher products sold in the U.S. We have initiated the relevant patent application process and have commissioned domestic distributors to provide trial cardiac organoid products (logistics costs only).

Cardiac organoids formed through small molecule induction of iPSCs can autonomously contract within six days. The produced cardiac organoids 100% express the following cardiac-specific markers:

• a-Actinin: α-actinin ensures the stability and function of cardiomyocytes by maintaining sarcomere structure, transmitting contractile force, participating in signal transduction, and facilitating cell adhesion.
• TNN2: TNN2 ensures normal cardiac function by regulating myocardial contraction, transmitting calcium signals, and maintaining structural stability.
• CX-43: CX-43 forms gap junctions that promote the transmission of electrical signals between cardiomyocytes, ensuring synchronized contraction and normal rhythm of the heart.
• DESMIN: desmin forms the cytoskeleton, providing mechanical support and ensuring the structural stability and proper alignment of cardiomyocytes. It also participates in intracellular signal transduction, maintaining cellular integrity and elasticity, helping the heart resist mechanical stress and injury.

Cardiac organoids derived from human iPSCs perfectly replicate the developmental process and structural functions of the human heart. Compared to Drosophila and animal models, they offer advantages of being faster, more accurate, and highly efficient. Our products have been utilized by university users (UPenn) to test whether newly discovered mechanosensitive genes in Drosophila have similar expression and functions in humans. Preliminary experimental results indicate that the candidate genes discovered in Drosophila exhibit homologous spatiotemporal expression during human heart development, providing an excellent experimental model for the development of cardiac disease drugs. 

The Na⁺/K⁺ currents of cardiomyocytes form the basis of cardiac electrophysiological activity, with the main characteristics including:

• Na⁺ Influx: Sodium channels open, allowing Na⁺ to enter the cell, causing depolarization and triggering contraction.
• K⁺ Efflux: Potassium channels open, allowing K⁺ to leave the cell, causing repolarization and preparing for the next action potential.
• Ion Gradient and Membrane Potential: The Na⁺/K⁺ current maintains the ion gradient and membrane potential, ensuring normal electrical activity and heart rhythm.

Our cardiomyocytes exhibit the typical characteristics of Na⁺/K⁺ currents, ensuring the normal electrophysiological function of cardiomyocytes and the effective pumping function of the heart.